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使用檢測試劑盒一般會遇到哪些問題？
發(fā)布時間： 2022-12-23 點擊次數(shù)： 669次檢測試劑盒保持了傳統(tǒng)ELISA法的快速、靈敏、簡便、載體易于標準化等優(yōu)點，并讓實驗過程更簡單更輕松，我司為生命科學領域產(chǎn)品的主要供應商之一，好品質(zhì)敢承諾，所有產(chǎn)品有任何質(zhì)量問題，全部可免費包換（除人為因素外）。實驗原理：檢測試劑盒應用雙抗體夾心法測定標本中待測物質(zhì)水平。用純化的待測物質(zhì)抗體包被微孔板，制成固相抗體，往包被單抗的微孔中依次加入待測物質(zhì)，再與HRP標記的待測物質(zhì)抗體結(jié)合，形成抗體-抗原-酶標抗體復合物，經(jīng)過洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的待測物質(zhì)呈正相關。用酶標儀在450nm波長下測定吸光度（OD值），通過標準曲線計算樣品中待測物質(zhì)濃度。檢測試劑盒實驗標本搜集：1、標本搜集后盡早進行提取，提取按相關文獻進行，提取后應盡快進行實驗，若不能馬上進行實驗，可將標本放于-20℃保存，但應防止反復凍融。2、血清：運用不含熱原的試管，操作過程中防止任何細胞刺激，搜集血液后，3000轉(zhuǎn)離心10分鐘將血清和紅細胞迅速小心腸別離。3、血漿：3000轉(zhuǎn)離心30分鐘取上清。4、細胞上清液：3000轉(zhuǎn)離心10分鐘去除顆粒和聚合物。5、組織勻漿：將組織加入適量生理鹽水搗碎，3000轉(zhuǎn)離心10分鐘取上清。6、保存：假如樣本搜集后不及時檢測，請按一次用量分裝，凍存于-20℃，防止反復凍融，在室溫下解凍并保證樣品均勻地充沛解凍。檢測試劑盒常見問題解析：1、加樣量紛歧致？解決方法：樣品稀釋前應充沛混勻，盡可能運用同一移液器并裝緊吸嘴。2、樣品數(shù)量多少紛歧，加樣時刻有長有短？解決方法：重復某一樣品時，加樣時刻盡可能與初次接近。3、保溫時刻紛歧致，洗刷條件紛歧致，操作人員紛歧致？解決方法：重復測定標本，操作條件、人員等應盡可能與前次保持一致，以掃除這些要素造成的紛歧致的可能性。
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